Standard fluorochrome in use are identical to FACS Calibur. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Proper Attachment of Fluidics Lines, Figure 4-1. endstream endobj 2781 0 obj <>stream Figure 1-1. If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri⢠C6 Flow Cytometer Optical Filter Guide . Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. With minor signs of normal wear and tear. �e�I�c~w1���0 ��� Came with note that it needs repair. !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is â3-blue 1-redâ with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. The BD Accuri⢠C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&���d7�.� ��L�q����5�H=�Y�pQAں��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� �! BD Accuri⢠C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri⢠C6 Software User Guide; BD CSampler⢠Accessory Installation Guide; BD CSampler⢠Software User Guide The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Accuri C6 consumables, manuals, etc. %%EOF BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Instructional video on the basics of using BYU's Accuri C6 cytometer. This includes microfuge tubes and tubes made of polystyrene and polypropylene. Accuri C6 consumables, manuals, etc. Available to trained users with online booking. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream endstream endobj startxref In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. 3. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Press the Power button on the BD Accuri C6 instrument face panel. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. Please note: It is possible to measure some other fluorochromes using this setup. Accuri C6 is a digital PC based bench top analyser, 2 laser system. Installation Dialog Box .....80 Figure C-3. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Flow cytometry within reach The analytical power and versatility of todayâs laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Includes all accessories shown in pictures. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . For Research Use Only. %PDF-1.6 %���� Accuri⢠C6 Plus BD Biosciences. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). ?���[��? BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Automated Preventive Maintenance Notification Window, Figure 4-4. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. 2774 0 obj <> endobj Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Manuals and User Guides for BD Accuri C6. The instrument will begin a 5 minute auto start cycle. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. BD Accuri⢠C6 Compensation Calculator (Microsoft® Excel®) BD Accuri⢠C6 Flow Cytometer Optical Filter Guide . Click here to view the BD Fluorescence Spectraviewer.. This manual provides information on cytometer requirements and specifications, setup, and maintenance. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ⢠software and FlowJo ⢠software. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. So the choice should be addressed by the kind of flow experiments you plan to do. x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. 661085). The BD Accuri⢠C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. Even more colors to choose from with selectable lasers and filter replacement. Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding endstream endobj 2779 0 obj <>stream endstream endobj 2778 0 obj <>stream 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H Click here to view the C6 software user guide. Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Overall very good cosmetic condition. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1.
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